Tatyana Dobreva, Ph.D. Thesis Defense

https://caltech.zoom.us/j/89893552024

My research focuses on developing experimental and computational tools to probe and manipulate cellular transcriptomes in the context of human health and disease. For probing cellular transcriptome states, I have leveraged single-cell RNA sequencing (scRNA-seq) to understand human immune variability, characterize cell-type specific biases of multiple viral variants within an animal, and assess temporal immune response in the brain to delivery of genetic cargo via an adeno-associated virus (AAV). In the context of manipulating cells, I have made progress on tools for exporting RNA extracellularly and engineering of a transcription factor for modulating macrophage state.