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Frontiers in Chemical Biology Seminar

Wednesday, February 5, 2020
4:00pm to 5:00pm
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Noyes 153 (J. Holmes Sturdivant Lecture Hall)
Engineered Protein Switches for Studying Dynamic Cellular Processes
Dustin Maly, Professor and Raymon E. and Rosellen M. Lawton Distinguished Scholar in Chemistry, Department of Chemistry and Biochemistry, University of Washington,

Synthetic protein switches that can be controlled with user-defined inputs are powerful tools for studying and controlling dynamic cellular processes. This talk will focus on the development and use of small molecule-regulated protein switches that rely on intramolecular autoinhibition. Efforts to engineer a small molecule-regulated protein switch that rapidly and dose-dependently controls the activity of the Cas9 endonuclease will be described. Furthermore, the use of our engineered Cas9 variant to gain insight into the kinetics and dose-dependence of Cas9-mediated DNA cleavage and subsequent repair will be presented. Finally, I will describe strategies for engineering small molecule-regulated protein switches that display sophisticated behaviors.

For more information, please contact Annette Luymes by phone at x6016 or by email at [email protected].